Date and time
Friday, August 7, 2020
10:30am - 12:30pm
Location

Zoom Meeting (Information posted at the end of the announcement)

Engineering Arterial Substitutes that Recapitulate Vessel Microstructure and Mimic Native Physiological Responses

Engineering small caliber (< 6mm) arterial grafts remains an unsolved problem. Current synthetic and autologous grafts suffer from short and long-term limitations including decreased patency rates, risk of bacterial infection, and compliance mismatching that results in neointimal hyperplasia. Tissue engineering is seen as a solution; however, a true arterial replacement remains elusive. Despite the numerous publications that have appeared over the last 25 years, most reported strategies mimic functional and structural arterial properties to a limited extent. Furthermore, these strategies require long maturation times before implantation and carry the risk of failure in patients, who are often elderly with multiple comorbidities. Our central hypothesis was that living arterial substitutes that display normal physiological responses after in vivo implantation can be engineered through the controlled assembly of vascular cells and free-standing collagen sheets of controlled fibril orientation in a manner that recapitulates native vessel microstructure.

We first present a scalable and continuous strategy for generating strong, free-standing, ultrathin, and centimeter-wide collagen sheets with controlled anisotropy using a flow-focusing approach. This strategy represents the first of its kind to generate anisotropic collagen sheets with control over nano- and macro-molecular properties. Next, controlled assembly of vascular cells and free-standing collagen sheets allowed us to design living blood vessels that recapitulated the arterial wall microstructure, and through structural, mechanical and biological characterization confirmed mimicry of native physiologic properties. We believe that the scalable fabrication schemes, and thorough characterization techniques, presented here will serve as a strong reference for future blood vessel tissue engineering efforts.
 
Thesis Supervisor:
Elliot L. Chaikof, MD, PhD
Johnson and Johnson Professor of Surgery, HMS; Chair of the Department of Surgery and Surgeon-in-Chief, BIDMC

Thesis Committee Chair:
Elazer R. Edelman, MD, PhD
Edward J. Poitras Professor in Medical Engineering and Science, MIT; Professor of Medicine, HMS

Thesis Reader:
Chad Cowan, PhD
Assistant Professor of Medicine, HMS

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Zoom information

Topic: David Miranda-Nieves: MEMP PhD Thesis Defense

Time: Friday, August 7, 2020 10:30 AM Eastern Time (US and Canada)

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