Zoom Meeting (Information posted at the end of the announcement)
Modular magnetic relaxation nanomaterial biosensor platform for localized, integrative chemical monitoring
The objective of this work was to develop an implantable diagnostic medical device for the tracking of temporally-varying disease chemical biomarkers. Continuous measurement of critical biomarkers provides a deeper understanding of physiology in response to stimulus, stress, or episodic and acute events. Stratification of patient phenotype can be based on the time course of biomarkers following such stimuli. Chronic disease surveillance in particular requires stable, long term in vivo sensors to realize such measurements. Current limitations in signal stability and inefficient transduction from opaque, heterogeneous biological environments hamper clinical implementation of sensing technologies. Cumulative exposure, colloidal nanoparticle, magnetic relaxometry assays coupled with single-sided NMR relaxometry for clinic and point-of-care, resource-limited settings are a promising technology for in vivo biosensing. Through a multidimensional combination of nanoscience, medical device development, magnetic relaxation measurement, and hydrogel design, this thesis endeavors to improve the diagnostic armamentarium of modular biosensors. Multicomponent populations of layered particles were designed to stably measure the presence of clinically-relevant biomolecules over the course of months. Reading the irreversible interaction of these particles with the biochemical markers they are tuned against by magnetic relaxation allows for wireless, non-invasive measurement providing insight into real-time results over varying timescales. Here we show that by fundamental advancements in the exploration of nanomaterial properties, chemical conjugation strategies, and device engineering parameters, the kinetic and signal amplitude performance of a switch-based dosimeter was improved by nearly an order of magnitude. The simultaneous investigation of localization and data extraction methods for implanted devices and the characterization of hydrogel material diffusion barriers also serves to advance the translation of magnetic relaxation biosensors beyond a benchtop system toward a clinically relevant, implantable platform technology. The implications of this work will help guide the personalized medicine campaign in biosensing and provide valuable insights into the next generation of diagnostic management of chronic disease.
Thesis Supervisor:
Michael J. Cima, PhD
David H. Koch Professor of Engineering, MIT
Thesis Committee Chair:
Angela M. Belcher, PhD
James Mason Crafts Professor of Biological Engineering and Materials Science and Engineering, MIT
Thesis Readers:
Elazer R. Edelman, MD, PhD
Edward J. Poitras Professor in Medical Engineering and Science, MIT
Professor of Medicine, HMS
Sangeeta Bhatia, MD, PhD
John J. and Dorothy Wilson Professor of Engineering, Institute for Medical Engineering and Science and Department of Electrical Engineering and Computer Science, MIT
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Zoom invitation –
Josh Murdock is inviting you to a scheduled Zoom meeting.
Topic: R. Joshua Murdock Thesis Defense
Time: Wednesday, July 21, 2021 10:00 AM Eastern Time (US and Canada)
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